| Description |
EzWay™ Annexin V-FITC Apoptosis Kit can identify apoptosis at an earlier stage than DNA-based assays such as TUNEL. Annexin V is a calcium-dependant phospholipid binding protein that has a high affinity for phosphatidylserine (PS), a plasma membrane phsopholipid. In non-apoptotic cells, most PS are localized at the inner layer of the plasma membrane, but after inducing apoptosis, PS are exposed to the extracellular environment, and then Annexin V binds to PS. Cells that stain positive for Annexin V-FITC (green) and negative for PI are undergoing apoptosis. Cells that stain positive for both Annexin V-FITC (green) and PI (red) are either in the end stage of apoptosis, are undergoing necrosis, or are already dead. Cells that stain negative for both Annexin V-FITC and PI are alive and not undergoing measurable apoptosis. |
| Specification |
• Utilizes FITC-conjugated Annexin V protein for detection of cells undergoing apoptosis
• Annexin V FITC binds to the membranes of apoptotic cells
• Detection by flow cytometry or fluorescence microscopy |
| Components |
• Annexin V-FITC
• 5X Binding buffer
• Propidium Iodide (PI) |
| Citation |
■ Corosolic acid reduces 5‑FU chemoresistance in human gastric cancer cells by activating AMPK
JB Park, JS Lee, MS Lee, EY Cha, S Kim , JY Sul
Molecular medicine reports, 2018
■ Tumor necrosis factor α-converting enzyme inhibitor attenuates lipopolysaccharide-induced reactive oxygen species and mitogen-activated protein kinase expression in human renal proximal tubule epithelial cells
EH Bae, IJ Kim, HS Choi, HY Kim, CS Kim, SK Ma, IS Kim, SW Kim
Korean J Physiol Pharmacol, 2018
■ The quinone-based derivative, HMNQ induces apoptotic and autophagic cell death by modulating reactive oxygen species in cancer cells
EB Lee, MG Cheon, J Cui, YJ Lee, EK Seo, HH Jang
Oncotarget, 2017
■ Trifloxystrobin induces tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis in HaCaT, human keratinocyte cells
Y Jang, AY Lee, SH Chang, SH Jeong, KH Park
Drug and Chemical Toxicology, 2016
■ Nicotine-Induced Apoptosis in Human Renal Proximal Tubular Epithelial Cells
CS Kim, JS Choi, SY Joo, EH Bae, SK Ma, JU Lee
PLoS One, 2016
■ Cytotoxic Compounds from Juglans sinensis Dode Display Anti-Proliferative Activity by Inducing Apoptosis in Human Cancer Cells
YJ Lee, J Cui, J Lee, AR Han, EB Lee, HH Jang
Molecules, 2016
■ 5-lipoxygenase mediates docosahexaenoyl ethanolamide and N-arachidonoyl-L-alanine-induced reactive oxygen species production and inhibition of proliferation of head and neck squamous cell carcinoma cells
SW Park, JH Hah, SM Oh, WJ Jeong, MW Sung
BMC Cancer, Article number: 458 (2016)
■ Chlorpyrifos induces NLRP3 inflammasome and pyroptosis/apoptosis via mitochondrial oxidative stress in human keratinocyte HaCaT cells
Y Jang, AY Lee, SH Jeong, KH Park, MK Paik, NJ Cho
Toxicology, 2015
■ Cold-inducible RNA-binding protein, CIRP, inhibits DNA damage-induced apoptosis by regulating p53
HN Lee, SM Ahn, HH Jang
Biochemical and Biophysical Research Communications 2015
■ Saururus chinensis Baill induces apoptosis through endoplasmic reticulum stress in HepG2 hepatocellular carcinoma cells
AY Lee, YA Han, JE Kim, SH Hong, EJ Park, MH Cho
Food and Chemical Toxicology 2015
■ Dielectric Barrier Discharge Plasma Efficiently Delivers an Apoptotic Response in Human Monocytic Lymphoma
N Kaushik, N Kumar, CH Kim, NK Kaushik, EH Choi
Plasma Processes and Polymers, 2014
■ CXCR2 and its related ligands play a novel role in supporting the pluripotency and proliferation of human pluripotent stem cells
JH Jung, SJ Lee, JH Kim, SH Lee, HJ Sung, J An
Stem Cells and Development, 2014
■ Altered Antioxidant System Stimulates Dielectric Barrier Discharge Plasma-Induced Cell Death for Solid Tumor Cell Treatment
NK Kaushik, N Kaushik, D Park, EH Choi
PLOS ONE, 2014
■ Epigenetic modification of Nrf2 in 5-fluorouracil-resistant colon cancer cells: involvement of TET-dependent DNA demethylation
KA Kang, MJ Piao, KC Kim, HK Kang, WY Chang
Cell Death & Disease, 2014
■ Inhibition of C2C12 myotube atrophy by a novel HSP70 inducer, celastrol, via activation of Akt1 and ERK1/2 pathways
T Gwag, K Park, E Kim, C Son, J Park, T Nikawa, I Choi
Archives of Biochemistry and Biophysics 2013
■ Amurensin G induces autophagy and attenuates cellular toxicities in a rotenone model of Parkinson's disease
H Ryu, WK Oh, IS Jang, J Park
Biochemical and Biophysical Research Communications 2013
■ Macrophage-stimulating protein attenuates hydrogen peroxide-induced apoptosis in human renal HK-2 cells
KE Lee, EY Kim, CS Kim, JS Choi, EH Bae, SK Ma
European Journal of Pharmacology 2013
■ Macrophage-stimulating protein attenuates gentamicin-induced inflammation and apoptosis in human renal proximal tubular epithelial cells
KE Lee, EY Kim, CS Kim, JS Choi, EH Bae, SK Ma
Biochemical and Biophysical Research Communications 2013
■ A MAP kinase pathway is implicated in the pseudohyphal induction by hydrogen peroxide in Candica albicans
K Srinivasa, J Kim, S Yee, W Kim, WJ Choi
Molecules and Cells, 2012 |
