Forensic Identification
| Product Name | EzWay Direct F PCR Kit |
|---|---|
| Cat. No. | K0569000 |
| Price | Please inquire |
| Size | 100T |
| Description |
|---|
A major limitation of Polymerase Chain Reaction (PCR)-based research and diagnostic and forensic tests is the inhibition of DNA polymerases by many substances like as anticoagulants (e.g., heparin, EDTA, sodium citrate) and heme group, proteins, polysaccharides, cell debris, and charged inorganic compounds, etc. Therefore, DNA is usually extracted from specimen according to phenol-chloroform method or by an alternative ‘silica-membrane filter’ method. However, this is time consuming and labor intensive and there is also a risk of introducing contamination during the preparation. |
| Specification |
• Direct PCR from buccal or blood FTA disc, blood or hair lysate without any DNA purification steps just by immersing the FTA punch or pipetting lysate into PCR mixture |
| Components |
• EzWay Direct F Buffer Mix 100T (25uL PCR) |
| Platform |
• Applied Biosystems 310 Genetic Analyzer filled with POP-4 & 36cm capillaries |
| Applications |
Human identification with a forensic sample |
| Citation |