PCR Reagent

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Product Name

Taq DNA Polymerase Kit II (MgCl2 Free Reaction Buffer)
Cat. No.

K0561004
Price Please inquire
Size 250U
Manual/Data Sheet Inquiry
Description
Taq DNA Polymerase is the enzyme from the cloned Thermus aquaticus expressed in E.coli. This unmodified enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5'→3' direction in the presence of magnesium and also possesses a 5'→3' exonuclease activity.
Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions.
Specification
• Ultrapure, thermostable recombinant enzyme
• Highly thermostable: 10min at 97°C, 60min at 94°C
• 5'→3' exonuclease activity
• Extra A addition activity
• Amplification range:
- Lambda DNA: up to 6Kb (Max. 12Kb)
- Human genomic DNA: up to 2Kb (Max. 4Kb)
• Composition of Taq polymerase and Taq PCR buffer without MgCl2
• No Nuclease, RNase, Protease contamination
Components
• Taq DNA Polymerase II, 250U
• 10X Taq PCR Buffer II (MgCl2 free)
• MgCl2 Stock solution
• dNTP Mix (2.5mM each)
• 10X Loading Buffer
• Nuclease Free Water
• 4X Magic Buffer
Citation
 
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